MK801 regulates the expression of key osteoarthritis factors in osteoarthritis synovial fibroblasts through complement C5

Osteoarthritis is currently one of the most common chronic diseases. As life expectancy increases, its prevalence and incidence are expected to rise. At present, more evidences prove correlation between complement system osteoarthritis (OA). This study aims investigate C5's influence on effect MK801 synovial fibroblasts (OA-SFs). We used IL-1b induce OA-SFs derived from mice obtain OA-SFs. And we performed RT-PCR Western Blot assays evaluate expression levels associated mRNA protein. The alteration MAC cell membrane was evaluated by immunofluorescence assay. related inflammatory factors ELISA experiment. could significantly inhibit (OA) marker factors, such as: attack complex (MAC), tumor necrosis factor-α (TNF-α) matrix metalloproteinase-13 (MMP13). Meanwhile, can C5 (C5) in Immunofluorescence assay showed that inhibited MK801. nucleo-plasmic separation experiment demonstrated activation Nuclear factor-κB (NF-κB) signaling pathway Futhermore, koncking down reversed inhibition factors. These results illustrated two points: first, generation release inflammation through C5; second: NF-κB